This project will combine CRISPRi genome editing with single-nucleus RNA-sequencing to assess the functional role of regulatory elements. We will also develop an analytical pipeline to integrate the CRISPRi screening with chromosome conformation Hi-C data. The CRISPRi screening method and the integrative analytical pipeline developed here would have broad applications for studying regulatory DNA sequences in any cell type.
CRISPRi screening of regulatory regions using snRNA-seq readout and integration with Hi-C data
Chief Investigator:
Co-Investigator Team:
UNSW BioMedical Machine Learning Lab (BML),, Graduate School of Biomedical Engineering, Level 1, Room 1002, Biological Sciences Building (E26)