Single cell somatic mutations

Detecting somatic variation in individual cells, or cells sequenced in a spatial context will enable the detection of cell-level clonotypes in cancer or autoimmune disease. Generating single RNA-sequence data on the same cells will enable the clones to be linked to biological processes, function, and behaviour of the cells. This project in collaboration with Professor Hanlee Ji (Stanford University) is developing a new method to detect both somatic mutations and RNA in individual cells and cells sequenced in an in situ setting across three systems: brain cancer, head and neck cancer, and B-cells in autoimmune diseases.